DNA vector and enzymatic manufacturing process


Disruptive, proprietary enzymatic DNA vector

At Touchlight, we are disrupting the decades-old technology of DNA manufacture to advance genetic medicine. We have developed a novel, synthetic DNA vector, known as doggybone DNA or dbDNA™ and enzymatic manufacturing process, which enables us to produce DNA at unprecedented speed, scale and purity.

Named after its schematic structure, doggybone DNA or dbDNA™ is a minimal, linear, double stranded and covalently closed DNA construct. dbDNA™ can encode long, complex, or unstable DNA sequences, eliminates bacterial sequences and has a strong expression profile.

Doggybone DNA animation
Enzymatic DNA

Making DNA using enzymes

Traditionally, DNA is manufactured using E.coli fermentation. This method for manufacturing plasmid DNA (pDNA) is inherently limited in terms of speed, safety, cost, capacity, and scalability.

As an alternative to pDNA production, we have developed synthetic DNA manufacturing using an in vitro enzymatic process.

By moving away from restrictive biological approaches and instead using enzyme biochemistry, we are making DNA simple – we have eliminated the need for bacterial sequences, including antibiotic resistance genes.

Thanks to this fundamental shift from using a complex biological system to a simpler, enzymatic approach, our dbDNA™ manufacturing has numerous benefits compared to pDNA:

Rapid enzymatic DNA technology

Enzymatic manufacturing enables multi-gram GMP DNA production in weeks rather than months

Scalable enzymatic DNA technology

Manufactured using benchtop equipment with a significantly smaller footprint than fermentation-based production

Mobile enzymatic DNA

dbDNA™ technology is highly portable and amenable to technology transfer

Optimal enzymatic DNA vector
Optimal vector

dbDNA™ can encode long, complex, unstable sequences, eliminates bacterial sequences, and has a strong expression profile


>5x faster than pDNA manufacture

Our proprietary enzymatic DNA manufacturing platform enables multi-gram DNA production in weeks, rather than the months taken to make plasmid DNA using traditional E.coli fermentation methods.

Timeline enzymatic DNA vs plasmid DNA comparison 4 Vs
Timeline enzymatic DNA vs plasmid DNA comparison 3
dbDNA process:
  1. pDNA production
  2. dbDNA production
  3. dbDNA release
Timeline enzymatic DNA vs plasmid DNA comparison
Timeline enzymatic DNA vs plasmid DNA comparison 2
Plasmid process:
  1. Optimisation
  2. MCB
  3. Development
  4. pDNA production
  5. pDNA release

Revolutionising DNA manufacture

With our proprietary enzymatic DNA production process, we can rapidly manufacture multiple grams of DNA using benchtop, disposable equipment.

Template DNA denatured

The starting, circular DNA template, containing the sequence of interest and bacterial backbone, is chemically denatured.

Rolling circle amplification

Addition of Φ29 DNA Polymerase and primers initiates rolling circle amplification, producing long, linear, double stranded concatemers.

Cleave and covalently close

Protelomerase binds the linear DNA at specified sites and performs a cleavage-joining reaction to produce dbDNA™.

First purification

The mixture is partially purified to remove low molecular weight impurities and enzymes using column chromatography and buffer exchange.

Backbone digested

Targeted restriction enzymes are added to selectively cut and digest the bacterial backbone DNA sequences.

Backbone removed

Exonucleases, to which dbDNA™ is resistant, remove the open fragments of backbone DNA sequences, producing dNMPs.

Second purification

The mixture is purified to remove the dNMPs and processing enzymes using column chromatography and buffer exchange.

Formulation and testing

The dbDNA™ is finally formulated, filtered, dispensed under laminar air flow, and quality checked prior to release.

Our platform is built on rigorous research

Find out more about our doggybone DNA (dbDNA™) vector and enzymatic manufacturing process in our knowledge centre

Read our research
doggybone enzymatic DNA manufacturing process

We address a broad market

Our versatile dbDNA™ platform technology can be applied throughout the genetic medicine industry.

Find out more